Thumbnail Download File format File size Pixel type Dimensions License Contributed by
Mouse kidney section from Invitrogen's FluoCell prepared slide #3. The section is stained with Alexafluor 488 WGA, Alexafluor 568 phalloidin and DAPI, and is imaged as two series: an XYZ stack (25 planes), and an XZY version (46 planes) of the same zone.
mouse-kidney.zip Leica LIF 84,788,147 bytes uint16 512 x 512
71 focal planes
4 channels
CC0 PFID - ImagopĂ´le, Institut Pasteur
4D dataset of C. elegans embryo development up to 8-cell stage
EmbryoCE.zip TIFF 294,270,460 bytes ? 425 x 434
12 focal planes
CC0 Kevin O'Connell, NIDDK, National Institutes of Health
dub.zip Bio-Rad PIC 756,808,942 bytes uint8 768 x 512
85 time points
33 focal planes
CC0 William Mohler, Health Center, University of Connecticut-Farmington
embryo2.zip Openlab LIFF 59,092,227 bytes ? 672 x 512
33 time points
3 channels
CC0 NIDDK, National Institutes of Health
2chZT.zip Zeiss LSM 54,055,250 bytes uint8 400 x 300
19 time points
21 focal planes
2 channels
CC0 Zeiss
10-31_E1.zip Olympus Fluoview TIFF 50,556,723 bytes uint16 512 x 512
16 time points
21 focal planes
CC0 Timothy Gomez, Anatomy, UW-Madison
TAABA.zip Bio-Rad PIC 42,939,725 bytes uint8 512 x 512
45 time points
5 focal planes
CC0 -
dnasample1.zip Gatan DM3 25,689,710 bytes uint16 4096 x 4096 CC0 Materials Science & Engineering, UW-Madison College of Engineering
KEVIN2-3.zip PerkinElmer 23,911,099 bytes ? 672 x 512
2 time points
29 focal planes
3 channels
CC0 Kevin O'Connell, NIDDK, National Institutes of Health
NESb.zip TIFF 22,463,680 bytes uint8 1024 x 1024
10 time points
9 focal planes
CC0 -
MF-2CH-Z-T.zip ABD TIFF 16,163,765 bytes uint16 256 x 256
5 time points
4 focal planes
2 channels
5 fields
CC0 Andor Technology
mitosis-test.zip Image-Pro workspace 12,642,856 bytes uint8 256 x 256
7 time points
24 focal planes
2 channels
CC0 -
sdub.zip Bio-Rad PIC 5,742,740 bytes uint8 192 x 128
12 time points
9 focal planes
CC0 William Mohler, University of Connecticut - Farmington
wtembryo.zip QuickTime 5,523,978 bytes uint8 320 x 240
108 time points
3 channels
CC0 -
HEART.zip Image-Pro 3,296,110 bytes uint8 512 x 512
30 focal planes
CC0 -
kidney_TFl.zip Windows Bitmap 2,083,265 bytes uint8 384 x 384
9 focal planes
3 channels
CC0 Dennis Fan, Department of Mechanical and Materials Engineering, Florida International University
Blend Final IPLab 691,397 bytes uint8 650 x 515
3 channels
CC0 Scanalytics
Leica stack Leica 83,581 bytes uint8 256 x 256
3 focal planes
CC0 Wisconsin National Primate Research Center, UW-Madison
qdna1.zip Image Cytometry Standard 42,831 bytes uint8 256 x 256 CC0 Nico Stuurman, Vale Laboratory, University of California-San Diego
Gray-FLIM-datasets.zip Image Cytometry Standard 16,681,761 bytes Various Various CC0 Paul Barber, Oxford Institute for Radiation Oncology, University of Oxford
test3-DAPI-2-(387).zip Hamamatsu-NDPI 3,326,629 bytes uint8 3968 x 4864
3 channels
CC BY 4.0 Manuel Stritt

Test images (used for validation)

Thumbnail Download File Size File format License Contributed by
An AVI version of the T1 Head Renderings sample dataset from the ImageJ sample images. The dataset is a stack of 36 surface renderings of the "T1 Head" volume. It was created using the "Render Cine-mode" option in VolumeJ, using the default settings. The "T1 Head" is a T1 weighted MRI head dataset courtesy of Jeff Orchard. Voxels outside the head have been set to zero to improve file compression. This was done by subtracting 32 from each of the 129 slices.
test-avi.zip AVI 752,503 bytes CC0 Jeff Orchard and Wayne Rasband
test-eps.zip EPS 101,557 bytes CC0 Gabriel Einsdorf
test-gif.zip GIF 311,118 bytes CC0 Gabriel Einsdorf
test-ics.zip Image Cytometry Standard (ICS) 334,825 bytes CC0 Jan Eglinger
test-jpeg2000.zip JPEG 2000 200,179 bytes CC0 Gabriel Einsdorf
test-jpg.zip JPEG 102,382 bytes CC0 Gabriel Einsdorf
test-pict.zip QuickTime PICT 100,055 bytes CC0 Gabriel Einsdorf
test-png.zip Portable Network Graphics (PNG) 1,023,286 bytes CC0 Gabriel Einsdorf