[SCIFIO] opening tiff files with ImageJ2

Martin Höhne martin.hoehne at uk-koeln.de
Wed Jan 15 06:36:48 CST 2014


Dear list,

We have 16 bit Tiff files from a camera (from a Chemoluminescence detector. Fusion XL machine form Vilber Lourmat http://www.vilber.com/). The images can be opened fine in Fiji (open or drag&drop). However, importing via File>Import>BioFormats fails. I get this error:

"Sorry, there was a problem during import". The log file is posted at the end of this mail.

We are fine with the normal opening, however the ImageJ2 seems to try to open with the import function (2.0.0.0 beta7.6). I get this message:
"io.scif.img.ImgIOExceprion: java.io.EOFException: Attempting to read beyond end of file" 

The image contains some metadata I can´t read when opening with ImageJ, but only when opening the file with the Fusion software from Vilber.
For the attached file the metadata is at the end of this posting as well.

The metadata is not so important, but it would be great if the images could be opened with ImageJ2 as well.

Thank you
Martin

PS - I hope this ist he right list to post. If not, maybe someone could direct me to the best suited forum

System information:
Win7 64bit
Fiji, updatet 2014-01-15

Log from import:
Fiji(legacy mode) File>Import>BioFormats:
loci.formats.FormatException: loci.common.ReflectException: java.lang.reflect.InvocationTargetException
	at loci.formats.in.TiffJAIReader.openBufferedImage(TiffJAIReader.java:202)
	at loci.formats.in.TiffJAIReader.initFile(TiffJAIReader.java:162)
	at loci.formats.FormatReader.setId(FormatReader.java:1360)
	at loci.formats.DelegateReader.setId(DelegateReader.java:275)
	at loci.plugins.in.ImportProcess.initializeFile(ImportProcess.java:482)
	at loci.plugins.in.ImportProcess.execute(ImportProcess.java:146)
	at loci.plugins.in.Importer.showDialogs(Importer.java:141)
	at loci.plugins.in.Importer.run(Importer.java:79)
	at loci.plugins.LociImporter.run(LociImporter.java:81)
	at ij.IJ.runUserPlugIn(IJ.java:196)
	at ij.IJ.runPlugIn(IJ.java:160)
	at ij.Executer.runCommand(Executer.java:131)
	at ij.Executer.run(Executer.java:64)
	at java.lang.Thread.run(Thread.java:662)
Caused by: loci.common.ReflectException: java.lang.reflect.InvocationTargetException
	at loci.common.ReflectedUniverse.exec(ReflectedUniverse.java:125)
	at loci.formats.in.TiffJAIReader.openBufferedImage(TiffJAIReader.java:197)
	... 13 more
Caused by: java.lang.reflect.InvocationTargetException
	at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
	at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:39)
	at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:25)
	at java.lang.reflect.Method.invoke(Method.java:597)
	at ome.scifio.common.ReflectedUniverse.exec(ReflectedUniverse.java:317)
	at loci.common.ReflectedUniverse.exec(ReflectedUniverse.java:122)
	... 14 more
Caused by: java.io.EOFException
	at com.sun.media.jai.codec.SeekableStream.readFully(SeekableStream.java:329)
	at com.sun.media.jai.codec.SeekableStream.readFully(SeekableStream.java:306)
	at com.sun.media.jai.codec.SeekableStream.readUnsignedIntLE(SeekableStream.java:708)
	at com.sun.media.jai.codec.TIFFDirectory.readUnsignedInt(TIFFDirectory.java:524)
	at com.sun.media.jai.codec.TIFFDirectory.initialize(TIFFDirectory.java:289)
	at com.sun.media.jai.codec.TIFFDirectory.<init>(TIFFDirectory.java:125)
	at com.sun.media.jai.codecimpl.TIFFImage.<init>(TIFFImage.java:318)
	at com.sun.media.jai.codecimpl.TIFFImageDecoder.decodeAsRenderedImage(TIFFImageDecoder.java:112)
	... 20 more


Metadata of attached image:
Good Laboratory Practice:GLP

GENERAL INFORMATION
   Title: 
   Comments:
   Date:  Freitag, 13. Dezember 2013
   Time: 13:42:04
   Image name: C:\Users\nephrolab\Pictures\temp_western\mhoehne\eva24__1.TIF

   Software information: FusionCapt Advance  Solo 4 16.07

ACQUISITION PARAMETERS: 

Lens information:
   Iris aperture:  

Camera information:
   Integration time acquisition: : 0 min 50 sec 0 msec
   Acquisition mode: Serial/r/n      Image 1/10  
   Positive acquisition 
   Resolution used: Binning 2x2 
   Pixel depth 16 Bit per pixel  
   Software acquisition mode: Not defined 
   Vigneting filter : Aperture value 0.84
   Sensor area used: : (0, 0, 2048,2048)
   Configuration file used: C:\Users\nephrolab\Documents\Vilber Lourmat\nephrolab_settings\2x2 binning.prt
   Camera serial number: 275704
   Tray position:  Non applicable
   Application name: 2x2 binning
   Auto mode: User defined autoexposure coef 97%
   USB status: No USB detected
   Imaging system: FusionCapt Advance  Solo 4 16.07
   Camera serial number: 275704   - Firmware >2.0.0<
   USB codage: No darkroom
   Lens configuration: Non applicable
   RGB status: Not installed
   Skylight: Not installed

Image manipulation 

   Gray level inversion: No

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