[SCIFIO] opening tiff files with ImageJ2
Martin Höhne
martin.hoehne at uk-koeln.de
Wed Jan 15 06:36:48 CST 2014
Dear list,
We have 16 bit Tiff files from a camera (from a Chemoluminescence detector. Fusion XL machine form Vilber Lourmat http://www.vilber.com/). The images can be opened fine in Fiji (open or drag&drop). However, importing via File>Import>BioFormats fails. I get this error:
"Sorry, there was a problem during import". The log file is posted at the end of this mail.
We are fine with the normal opening, however the ImageJ2 seems to try to open with the import function (2.0.0.0 beta7.6). I get this message:
"io.scif.img.ImgIOExceprion: java.io.EOFException: Attempting to read beyond end of file"
The image contains some metadata I can´t read when opening with ImageJ, but only when opening the file with the Fusion software from Vilber.
For the attached file the metadata is at the end of this posting as well.
The metadata is not so important, but it would be great if the images could be opened with ImageJ2 as well.
Thank you
Martin
PS - I hope this ist he right list to post. If not, maybe someone could direct me to the best suited forum
System information:
Win7 64bit
Fiji, updatet 2014-01-15
Log from import:
Fiji(legacy mode) File>Import>BioFormats:
loci.formats.FormatException: loci.common.ReflectException: java.lang.reflect.InvocationTargetException
at loci.formats.in.TiffJAIReader.openBufferedImage(TiffJAIReader.java:202)
at loci.formats.in.TiffJAIReader.initFile(TiffJAIReader.java:162)
at loci.formats.FormatReader.setId(FormatReader.java:1360)
at loci.formats.DelegateReader.setId(DelegateReader.java:275)
at loci.plugins.in.ImportProcess.initializeFile(ImportProcess.java:482)
at loci.plugins.in.ImportProcess.execute(ImportProcess.java:146)
at loci.plugins.in.Importer.showDialogs(Importer.java:141)
at loci.plugins.in.Importer.run(Importer.java:79)
at loci.plugins.LociImporter.run(LociImporter.java:81)
at ij.IJ.runUserPlugIn(IJ.java:196)
at ij.IJ.runPlugIn(IJ.java:160)
at ij.Executer.runCommand(Executer.java:131)
at ij.Executer.run(Executer.java:64)
at java.lang.Thread.run(Thread.java:662)
Caused by: loci.common.ReflectException: java.lang.reflect.InvocationTargetException
at loci.common.ReflectedUniverse.exec(ReflectedUniverse.java:125)
at loci.formats.in.TiffJAIReader.openBufferedImage(TiffJAIReader.java:197)
... 13 more
Caused by: java.lang.reflect.InvocationTargetException
at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:39)
at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:25)
at java.lang.reflect.Method.invoke(Method.java:597)
at ome.scifio.common.ReflectedUniverse.exec(ReflectedUniverse.java:317)
at loci.common.ReflectedUniverse.exec(ReflectedUniverse.java:122)
... 14 more
Caused by: java.io.EOFException
at com.sun.media.jai.codec.SeekableStream.readFully(SeekableStream.java:329)
at com.sun.media.jai.codec.SeekableStream.readFully(SeekableStream.java:306)
at com.sun.media.jai.codec.SeekableStream.readUnsignedIntLE(SeekableStream.java:708)
at com.sun.media.jai.codec.TIFFDirectory.readUnsignedInt(TIFFDirectory.java:524)
at com.sun.media.jai.codec.TIFFDirectory.initialize(TIFFDirectory.java:289)
at com.sun.media.jai.codec.TIFFDirectory.<init>(TIFFDirectory.java:125)
at com.sun.media.jai.codecimpl.TIFFImage.<init>(TIFFImage.java:318)
at com.sun.media.jai.codecimpl.TIFFImageDecoder.decodeAsRenderedImage(TIFFImageDecoder.java:112)
... 20 more
Metadata of attached image:
Good Laboratory Practice:GLP
GENERAL INFORMATION
Title:
Comments:
Date: Freitag, 13. Dezember 2013
Time: 13:42:04
Image name: C:\Users\nephrolab\Pictures\temp_western\mhoehne\eva24__1.TIF
Software information: FusionCapt Advance Solo 4 16.07
ACQUISITION PARAMETERS:
Lens information:
Iris aperture:
Camera information:
Integration time acquisition: : 0 min 50 sec 0 msec
Acquisition mode: Serial/r/n Image 1/10
Positive acquisition
Resolution used: Binning 2x2
Pixel depth 16 Bit per pixel
Software acquisition mode: Not defined
Vigneting filter : Aperture value 0.84
Sensor area used: : (0, 0, 2048,2048)
Configuration file used: C:\Users\nephrolab\Documents\Vilber Lourmat\nephrolab_settings\2x2 binning.prt
Camera serial number: 275704
Tray position: Non applicable
Application name: 2x2 binning
Auto mode: User defined autoexposure coef 97%
USB status: No USB detected
Imaging system: FusionCapt Advance Solo 4 16.07
Camera serial number: 275704 - Firmware >2.0.0<
USB codage: No darkroom
Lens configuration: Non applicable
RGB status: Not installed
Skylight: Not installed
Image manipulation
Gray level inversion: No
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